Fig. 5

rgs3 impacts segmentation stage Ca²⁺ dynamics.

Zebrafish embryos injected with Fura-2 were oriented in a dorsal posterior view. Representative ratio images, pseudocolored with low Ca²⁺ represented by blue, and high Ca²⁺ represented by yellow/red (A–C,F–N). During somitogenesis, Ca²⁺ transients are identified as a local short-lived increase in intracellular Ca²⁺ levels. A region of interest (ROI) is noted by a dashed circle highlighting a representative Ca²⁺ transient (A–C). In the ROI from time 0s to time 15s, an increase in Ca²⁺ levels is observed (B) that subsides by time 30s (C). The number of transients as a function of developmental age (D). Table depicting the average number of Ca²⁺ transients per hour from 6 to 12 somite stage for each treatment (E). Representative ratio images of 5 somite stage (F), 7 somite (G) and 10 somite stage (H) wt embryos taken from Video S1. Representative ratio images of 5 somite (I), 7 somite (J) and 10 somite stage (K) rgs3 MOsa injected embryo taken from Video S2. Representative ratio images of 5 somite (L), 7 somite stage (M) and 10 somite stage (N) wnt5b MO injected embryo taken from Video S4. Red arrowheads indicate large Ca²⁺ transients in rgs3 morphant embryos (I–K) that are not observed in wt (F–H) or wnt5b morphant embryos (L–N).