FIGURE

Fig. 8

ID

ZDB-FIG-100616-40

Publication

Choudhuri et al., 2010 - Non-core subunit eIF3h of translation initiation factor eIF3 regulates zebrafish embryonic development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Development of trunk neuromast sensory organs fails in the eif3ha morphants. Shown are representative embryos after staining with Di-Asp at 3 dpf to identify neuormasts. A: The representative embryo demonstrates the normal distribution of neuromasts (bright yellow spots) in an uninjected wild-type (WT) embryo. B,C: This is compared with one of the representative embryos injected with either 3 ng of eif3ha SB morpholino (MO-3ha) as shown in B or 2-4 ng of eif3hb TB morpholino (MO-3hb) as shown in C. Note that at this dose, the severe brain defect did not occur using MO-3ha, although most of the trunk neuromasts are absent in the eif3ha morphants (90-95%, n ~ 100). In contrast, for the eif3hb morphants, neuromast formation appears normal even though the cardiac phenotype for this morphant begins to appear (indicated by the white arrow).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO2-eif3hb AB/TU + MO3-eif3ha
Knockdown Reagents:	MO2-eif3hb MO3-eif3ha
Observed In:	pericardium posterior lateral line neuromast
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.