FIGURE

Fig. 5

ID

ZDB-FIG-100616-37

Publication

Choudhuri et al., 2010 - Non-core subunit eIF3h of translation initiation factor eIF3 regulates zebrafish embryonic development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Each of the eif3h morphants show distinct embryonic phenotypes. B,C,E,F: Shown are representative morphant phenotypes obtained by injecting either TB or SB morpholino (MO) for eif3ha (3ha-TB or 3ha-SB) at 1 dpf (B,C) and for eif3hb (3hb-TB or 3hb-SB) at 2 dpf (E,F). A,D: In each case, an uninjected wild-type (WT) control is also included. In panel A, different regions of the normal brain in a WT embryo at 24 hpf are shown: eye (E), forebrain (FB), midbrain (MB), midbrain-hindbrain boundary (MHB), and hindbrain (HB) as indicated by different colored arrows. For the eif3ha morphants (~100%, n ~ 200), after injection of either TB MO (B) or SB MO (C), gross disruption of all the brain regions are shown using brackets. For the eif3hb morphants (90-95%, n ~ 200) at 2 dpf obtained with either TB MO (E) or SB MO (F), specific defects in the form of edema seen around the heart region (H) and regression of yolk stalk (YS) are indicated with the red and green arrows, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO2-eif3ha AB/TU + MO2-eif3hb AB/TU + MO3-eif3ha AB/TU + MO3-eif3hb
Knockdown Reagents:	MO2-eif3ha MO2-eif3hb MO3-eif3ha MO3-eif3hb
Observed In:	brain brain development extension pericardium
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.