Amino acid sequence alignment of FGF10 proteins and comparison of fgf10 and tbx5 expression in the zebrafish embryo. (A) Alignment of deduced FGF10 protein sequences from zebrafish (Danio rerio, Dr), chick (Gallus gallus, Gg), mouse (Mus musculus, Mm), and human (Homo sapiens, Hs). Identical amino acids are indicated by dots, and gaps by dashes. The zebrafish sequence shows homology to that of chick (44.7%), mouse (42.7%) and human (41.7%). The GenBank accession number for zebrafish fgf10 is AF544025. (B-G) All panels show the dorsal view of zebrafish embryos with anterior to the top, excluding F and G. Whole-mount in situ hybridization staining of wild-type zebrafish embryos during pectoral fin development. fgf10 (B,D,F) and tbx5 (C,E,G) appear to be expressed in a similar spatial and temporal pattern during pectoral fin initiation and outgrowth. (B,C) fgf10 and tbx5 are expressed in the LPM of the presumptive pectoral fin bud (arrowheads) at 24 hpf. (D,E) At 30 hpf fgf10 is expressed in the branchial arches, otic vesicle, and pectoral fin bud (arrowheads), while tbx5 is expressed in the dorsal eye, heart tube and pectoral fin bud (arrowheads). (F,G) Lateral view of pectoral fin buds of 36 hpf embryos showing fgf10 and tbx5 expression throughout the mesenchyme. Anterior is to the left.
|
