FIGURE

Fig. S4

ID

ZDB-FIG-100504-62

Publication

Hartnett et al., 2010 - Insulin-like growth factor-2 regulates early neural and cardiovascular system development in zebrafish embryos

Other Figures

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Fig. S4

IGF-2a and IGF-2b are required for the development of anterior neural structures during zebrafish gastrulation and play anti-apoptotic roles during segmentation. (A, B) Knockdown of igf-2a or igf-2b results in a reduction of pax6.2 expression in the developing eye and midline region. (C, D) Knockdown of igf-2a or igf-2b causes a reduction of rx3 expression. (E) Knockdown of igf-2a results in an increase in apoptosis, particularly in the developing spinal cord. (F) Knockdown of igf-2b results in an increase in apoptosis, particularly in the anterior region of the embryo. Frequency of embryos displaying this staining pattern; A, 14/32; B, 8/20; C, 15/39; D, 14/33; E, 17/30; F, 12/33. A-D are views of the future anterior region and E, F are lateral views.

Expression Data

Genes:	pax6b rx3
Fish:	AB + MO1-igf2a AB + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Anatomical Terms:	axis forebrain neural keel optic primordium
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-igf2a AB + MO3-igf2b
Knockdown Reagents:	MO1-igf2a MO3-igf2b
Observed In:	apoptotic process neural keel spinal cord neural keel
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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