FIGURE

Fig. S5

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ZDB-FIG-100429-32

Publication

Ishida et al., 2010 - Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish

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Fig. S5

Expression of blastema and wound epithelium markers in the junb or junbl morphants at 3 hpa and 12 hpa. (A, C, F, H, K, M) junbl, (B, D, G, I, L, N) junb, and (E, J, O) dlx5a. Comparing with the normal expression of respective genes (A–E), the junbl morphants retained a nearly normal gene expression at 3 hpa (F, G) and 12 hpa (H–J). However, in the junb morphants, the expression of junb and dlx5a in the wound epithelium was not maintained at 12 hpa (arrows in N, O), while the junbl expression in the blastema normally occurred as in the junbl morphant. The down-regulation of junb expression at 12 hpa is similar to that obtained by the JNK inhibitor treatment (Fig. 3E). The scale bar represents 100 μm in (A).

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 340(2), Ishida, T., Nakajima, T., Kudo, A., and Kawakami, A., Phosphorylation of Junb family proteins by the Jun N-terminal kinase supports tissue regeneration in zebrafish, 468-479, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.