FIGURE

Fig. 7

ID

ZDB-FIG-100427-31

Publication

Binot et al., 2010 - Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells

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Fig. 7

The nkx6 genes are involved in producing/maintaining the endocrine progenitor pool. A–H: WISHs showing expression of sox4b at 18 somites (A–D) and of isl1 at 24 hpf (E–H) in control (A, E), nkx6.1 (B, F), nkx6.2 (C, G), and nkx6.1/nkx6.2 (D, H) morphants. I–L: Double fluorescent WISHs showing expression of isl1 and of all the pancreatic hormone genes together in control (I), nkx6.1 (J), nkx6.2 (K), and nkx6.1/nkx6.2 (L) morphants; Z-plane confocal images. All views are ventral with anterior part to the left. Scale: 20 μm. M–P: Schematic representations (lateral view) of the isl1-, sox4b-, and hormone-expressing cell populations in control and nkx6 morphants. Q: Quantitative analysis of the number of sox4b-expressing cells at 18 somites in control and nkx6 morphants. R: Quantitative analysis of the number of isl1-expressing cells at 24 hpf in control and nkx6 morphants (the horizontal line represents the mean). S: Quantitative analysis of the number of hormone-expressing and hormone-non-expressing isl1-cells at 24 hpf, expressed as percentages (100% being arbitrarily fixed as the number of cells in control embryos), in control and nkx6 morphants. The presented data are representative of at least three reproducible and independent experiments.

Expression Data

Genes:	isl1a sox4b
Fish:	AB AB + MO1-nkx6.1 + MO2-nkx6.1 AB + MO3-nkx6.2 + MO4-nkx6.2 AB + MO2-nkx6.1 + MO3-nkx6.2
Knockdown Reagents:	MO1-nkx6.1 MO2-nkx6.1 MO3-nkx6.2 MO4-nkx6.2
Anatomical Terms:	pancreas primordium endocrine cell pancreatic bud endocrine cell
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-nkx6.1 + MO2-nkx6.1 AB + MO3-nkx6.2 + MO4-nkx6.2 AB + MO2-nkx6.1 + MO3-nkx6.2
Knockdown Reagents:	MO1-nkx6.1 MO2-nkx6.1 MO3-nkx6.2 MO4-nkx6.2
Observed In:	endocrine cell pancreas primordium pancreatic bud
Stage Range:	14-19 somites to Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.