FIGURE

Fig. 5

ID

ZDB-FIG-100427-29

Publication

Binot et al., 2010 - Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Synergistic effects of nkx6 knockdown on alpha-cells. A–D: Ventral views of WISHs showing expression ofglucagon at 30 hpf in control morphants (A) and in morphants injected with 0.3 ng Mo2 6.1 (B), with 0.1 ng Mo1 6.2 (C), or with both simultaneously (D). Scale: 20 μm. E: Number of glucagon-expressing cells in these various morphants (the horizontal line represents the mean). The presented data are representative of at least two reproducible and independent experiments. F: Quantitative analysis of glucagon and insulin expression in 30 hpf embryos, expressed as percentages (100% being arbitrarily fixed as the number of cells in control embryos), following simultaneous injections of Mo2 6.1 and Mo1 6.2 at increasing concentrations. The presented data are representative of at least three reproducible and independent experiments.

Expression Data

Gene:	gcga
Fish:	AB AB + MO2-nkx6.1 AB + MO3-nkx6.2 AB + MO2-nkx6.1 + MO3-nkx6.2
Knockdown Reagents:	MO2-nkx6.1 MO3-nkx6.2
Anatomical Term:	pancreatic A cell
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-nkx6.1 + MO3-nkx6.2
Knockdown Reagents:	MO2-nkx6.1 MO3-nkx6.2
Observed In:	pancreatic A cell pancreatic B cell pancreatic bud
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 340(2), Binot, A.C., Manfroid, I., Flasse, L., Winandy, M., Motte, P., Martial, J.A., Peers, B., and Voz, M.L., Nkx6.1 and nkx6.2 regulate alpha- and beta-cell formation in zebrafish by acting on pancreatic endocrine progenitor cells, 397-407, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.