FIGURE

Fig. 3

ID

ZDB-FIG-100401-9

Publication

Christie et al., 2010 - Syk and Zap-70 function redundantly to promote angioblast migration

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Fig. 3

syk^HI:zap-70^HI morphants resemble vegfa morphants and lack activated ERK. (A) syk^HI:zap-70^HI Tg(fli1a:EGFP)^y1morphants have a complete suppression of ISV sprouting which is phenotypically similar to the ISV stalling observed in high dose vegfa morphants (B). While the artery of 20s wildtype (WT) embryos (arrow, C′) shows activated ERK (arrow, C″) comparable staged syk^HI:zap-70^HI morphants (D) show a strong downregulation of activated ERK (arrow D′ = artery, D″). The level of downregulation of activated ERK in the artery (arrow, E′) is similar to vegfa^HI morphants (E, E″). C′, D′, E′ are enlargements of GFP signal in the boxed region of C, D, E, respectively. C″, D″, E″ are enlargements of the αERK-P immunohistochemistry in the boxed regions of C, D, and E respectively.

Expression Data

Antibody:	Ab7-mapk
Fish:	y1Tg y1Tg + MO2-vegfaa y1Tg + MO1-syk + MO2-zap70
Knockdown Reagents:	MO1-syk MO2-vegfaa MO2-zap70
Anatomical Term:	vascular cord
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg + MO2-vegfaa y1Tg + MO1-syk + MO2-zap70
Knockdown Reagents:	MO1-syk MO2-vegfaa MO2-zap70
Observed In:	intersegmental vessel
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.