ZFIN Image: Christie et al., 2010, Fig. 3

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Figure Caption

Fig. 3 syk^HI:zap-70^HI morphants resemble vegfa morphants and lack activated ERK. (A) syk^HI:zap-70^HI Tg(fli1a:EGFP)^y1morphants have a complete suppression of ISV sprouting which is phenotypically similar to the ISV stalling observed in high dose vegfa morphants (B). While the artery of 20s wildtype (WT) embryos (arrow, C′) shows activated ERK (arrow, C″) comparable staged syk^HI:zap-70^HI morphants (D) show a strong downregulation of activated ERK (arrow D′ = artery, D″). The level of downregulation of activated ERK in the artery (arrow, E′) is similar to vegfa^HI morphants (E, E″). C′, D′, E′ are enlargements of GFP signal in the boxed region of C, D, E, respectively. C″, D″, E″ are enlargements of the αERK-P immunohistochemistry in the boxed regions of C, D, and E respectively.

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Acknowledgments

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Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.