FIGURE

Fig. 1

ID

ZDB-FIG-100128-12

Publication

Schütte et al., 2010 - Let It Flow: Morpholino Knockdown in Zebrafish Embryos Reveals a Pro-Angiogenic Effect of the Metalloprotease Meprin alpha(2)

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Fig. 1

Distribution of meprin α₁, α₂ and β in zebrafish tissues.

Immunofluorescence microscopy of cryosections from whole mount zebrafish, using specific peptide antibodies, revealed meprin α₁ in the brush border cells of intestinal epithelia (Ep) (A) and epidermis (B), whereas meprin α₂ was observed in the lamina propria mucosae (LP) only (C, D). Additionally, meprin β signals could be detected in the zebrafish intestine (Ep) and epidermis (Ed) (E, F respectively). To verify the efficiency of meprin knockdowns due to morpholino injection, we compared the fluorescence signal intensity in cryosections of wild type (G) and meprin α₂ deficient embryos (H). Evidently, the expression of meprin α₂ in the lamina propria (LP) of the intestine is largely decreased (G, H). Similar analyses of the meprin β morphant were not possible, due to the lethality within 24 hpf. (Ep: epithelium; Lu: lumen; LP: lamina propria; Ed: epidermis; De: dermis; scale bars: 25 μm; signal intensity was calculated with ImageJ V.1.41o).

Expression Data

Genes:	mep1a.1 mep1a.2 mep1ba
Antibodies:	Ab1-mep1a.1 Ab1-mep1a.2 Ab1-mep1b
Fish:	WT
Anatomical Terms:	brush border epithelial cell epidermis intestine brush border epithelial cell intestine mucus layer
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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