FIGURE

Fig. 4

ID

ZDB-FIG-100114-28

Publication

Codina et al., 2010 - Loss of Smyhc1 or Hsp90alpha1 function results in different effects on myofibril organization in skeletal muscles of zebrafish embryos

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Fig. 4

Effects of smyhc1 knockdown or hsp90α1 mutation on myosin thick filament organization in skeletal muscles of zebrafish embryos.

A–C. Anti-MyHC antibody (F59) staining shows the organization of thick filaments in trunk slow muscles of control (A), smyhc1 knockdown (B), or slo^tu44c mutant (C) embryos at 48 hpf. D–F. Anti-MLC antibody (F310) staining shows the organization of thick filaments in trunk fast muscles of control (D), smyhc1 knockdown (E), or slo^tu44c mutant (F) embryos at 72 hpf. Note, fast fibers project with a 30 degree angle with respect to the axial structure, whereas slow fibers project in parallel to the axial structure. G, H. Anti-MyHC antibody (F59) staining shows the rescue of thick filaments in smyhc1 knockdown zebrafish embryos co-injected with ef1a:smyhc1 DNA construct (G), or ATG-MO alone (H). Scale bar = 25 μm in A, 10 μm in G.

Expression Data

Antibodies:	Ab-F59 Ab-F310
Fish:	WT hsp90aa1.1^tu44c/tu44c WT + MO1-smyhc1
Knockdown Reagent:	MO1-smyhc1
Anatomical Terms:	fast muscle cell striated muscle myosin thick filament slow muscle cell striated muscle myosin thick filament
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hsp90aa1.1^tu44c/tu44c WT + MO1-smyhc1
Knockdown Reagent:	MO1-smyhc1
Observed In:	fast muscle cell striated muscle myosin thick filament slow muscle cell striated muscle myosin thick filament
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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