FIGURE

Fig. 5

ID

ZDB-FIG-100107-29

Publication

Pistocchi et al., 2009 - The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in the lateral line

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Fig. 5

prox1 loss of function affects development of functional hair cells in zebrafish lateral line neuromasts. Comparison of GFP expression between morphant and control embryos at 60 hpf shows no differences in mantle cells (A, B), progenitor cells (C, D) and hair cells (E, F) in different transgenic backgrounds, indicating that there is no effect of the prox1 morpholino in the specification of these cell types in the neuromast. (G, H) However, the vital stain Diasp indicates that almost 50% of the hair cells present in a neuromast are not functionally active. (I) Comparison of the percentage of GFP labeled hair cells that are co-labeled with Diasp in control and prox1 MO injected embryos. Scale bar = 10 micron.

Expression Data

Genes:	EGFP GFP
Fish:	s356tTg sqet20Et s356tTg + MO1-prox1a sqet20Et + MO1-prox1a tnks1bp1^y229Gt tnks1bp1^y229Gt + MO1-prox1a
Knockdown Reagent:	MO1-prox1a
Anatomical Terms:	neuromast somatic stem cell neuromast hair cell neuromast support cell unspecified
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s356tTg + MO1-prox1a sqet20Et + MO1-prox1a tnks1bp1^y229Gt + MO1-prox1a
Knockdown Reagent:	MO1-prox1a
Observed In:	neuromast somatic stem cell neuromast hair cell neuromast mantle cell neuromast support cell
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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