FIGURE

Fig. 4

ID

ZDB-FIG-100107-28

Publication

Pistocchi et al., 2009 - The zebrafish prospero homolog prox1 is required for mechanosensory hair cell differentiation and functionality in the lateral line

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Fig. 4

prox1 loss of function of does not affect PLL primordium cell number or PLL nerve development. (A, B) GFP labeled primordia migrating at 32 hpf in cldnb::GFP transgenic fish. The size of the primordium is not affected in morphant embryos in comparison to control embryos at the same developmental stage. (C, D, C′, D′) Acetylated tubulin immunostaining indicates that the lateral line nerve is not perturbed in morphant embryos (white arrows) while differentiated hair cell with their kinocilia (brown arrows C, D and in fluorescence at higher magnification C′, D′), are absent in morphant fish in comparison to control embryos. (E, F) prox1 MO injected embryos do not show increased cell death as indicated by the TUNEL assay, in comparison to control embryos. n, notochord; y, yolk. Scale bar = 15 micron in A, B, C, D, E, F and 3 micron in C′, D′.

Expression Data

Gene:	EGFP
Fish:	zf106Tg zf106Tg + MO1-prox1a
Knockdown Reagent:	MO1-prox1a
Anatomical Term:	primary posterior lateral line primordium
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prox1a zf106Tg + MO1-prox1a
Knockdown Reagent:	MO1-prox1a
Observed In:	neuromast hair cell kinocilium posterior lateral line nerve primary posterior lateral line primordium programmed cell death involved in cell development
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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