FIGURE

Fig. 5

ID

ZDB-FIG-091217-109

Publication

Hatler et al., 2009 - A gap junction connexin is required in the vertebrate left-right organizer

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Fig. 5

KV cells are specified in Cx43.4 morphants, though ciliary defects exists. Confocal fluorescence imaging (A, B) of KV cilia labeled with acetylated tubulin antibodies. Scale bar = 10 microns. (A) Uninjected, control embryos have well-developed cilia in a circular pattern, while (B) Cx43.4 morphants have disorganized, smaller ciliated KVs. Multiple fluorescence images of 10-somite stage KVs labeled with acetylated tubulin antibiodies were analyzed using ImageJ software (C, D) and data were plotted as means ± SEM. (C) The average number of cilia per KV was similar between uninjected and Cx43.4 morphant embryos, p = 0.13. (D) The average cilium in Cx43.4 morphant embryos was significantly shorter than a wild-type cilium, ∗p = 3 x 10^- 7.

Expression Data

Antibody:	Ab1-tuba
Fish:	WT WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Anatomical Term:	Kupffer's vesicle cilium
Stage:	10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gjc4b
Knockdown Reagent:	MO1-gjc4b
Observed In:	cilium assembly Kupffer's vesicle cilium
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 336(2), Hatler, J.M., Essner, J.J., and Johnson, R.G., A gap junction connexin is required in the vertebrate left-right organizer, 183-191, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.