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Fig. S2

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ZDB-FIG-091113-48

Publication

Joshi et al., 2009 - Amyloid precursor protein is required for convergent-extension movements during Zebrafish development

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Fig. S2

Effects of overexpression of APP₆₉₅ and its mutant constructs on embryonic development. Zebrafish embryos were injected with mRNA from APP₆₉₅ or indicated mutant constructs at 1–2 cell stage and observed at approximately 36 hpf. Embryos in column A were injected with 250 pg dosage and in column B with 500 pg dosage of mRNA encoding various APP constructs. Representative images are shown. Overexpression of each construct results in embryos with posteriorized phenotype with smaller head, smaller eyes (arrows) and thicker trunks (arrowheads). At either dose of mRNA, APP₆₉₅ is most effective in inducing the phenotype whereas CΔ18 is least effective. None of the constructs induce phenotypic changes below 200 pg dosage while most constructs, except CD18, cause severe phenotypic changes at 500 pg and above. APP₆₉₅ injected embryo showing posteriorization are shown at the top while representative images of embryos injected with indicated dose of various mutant constructs are shown below.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 335(1), Joshi, P., Liang, J.O., Dimonte, K., Sullivan, J., and Pimplikar, S.W., Amyloid precursor protein is required for convergent-extension movements during Zebrafish development, 1-11, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.