ZFIN Image: Joshi et al., 2009, Fig. S2

Image

Figure Caption

Fig. S2 Effects of overexpression of APP₆₉₅ and its mutant constructs on embryonic development. Zebrafish embryos were injected with mRNA from APP₆₉₅ or indicated mutant constructs at 1–2 cell stage and observed at approximately 36 hpf. Embryos in column A were injected with 250 pg dosage and in column B with 500 pg dosage of mRNA encoding various APP constructs. Representative images are shown. Overexpression of each construct results in embryos with posteriorized phenotype with smaller head, smaller eyes (arrows) and thicker trunks (arrowheads). At either dose of mRNA, APP₆₉₅ is most effective in inducing the phenotype whereas CΔ18 is least effective. None of the constructs induce phenotypic changes below 200 pg dosage while most constructs, except CD18, cause severe phenotypic changes at 500 pg and above. APP₆₉₅ injected embryo showing posteriorization are shown at the top while representative images of embryos injected with indicated dose of various mutant constructs are shown below.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 335(1), Joshi, P., Liang, J.O., Dimonte, K., Sullivan, J., and Pimplikar, S.W., Amyloid precursor protein is required for convergent-extension movements during Zebrafish development, 1-11, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.