Fig. 5

Met signaling is required for cerebellar proliferation. (A–C) Dorsal longitudinal confocal images (z-projections at maximum intensity) and (E–G′) single dorsal longitudinal confocal sections of posterior midbrain and anterior hindbrain of 24 hpf embryos of control (A, E and E′), Met morphants (B, F and F′) and Hgf double morphants (C, G and G′) illustrating total proliferation in the cerebellum (A–C), cerebellar proliferation within a confocal section marked by anti-PH3 antibody in red (E–G) and cerebellar nuclei within a confocal section counterstained with Sytox Green (E′–G′). Compared to controls (A), Met and Hgf double morphants (B and C) showed reduced total mitotic cells in cerebellar (cb) regions marked by brackets and outlined by the broken line. The graph (D) represents the quantitative analysis of total cerebellar proliferative cells, showing a highly significant decrease in global proliferation in the morphants compared to controls. The graph (H) represents the quantitative analysis of the mitotic index (mitotic cells/total cells) in the cerebellum showing a significant decrease in the mitotic index of morphants compared to controls. Bars represent the combined results of two independent experiments. n, the number of embryos assessed per experimental condition; asterisks in panels (D) and (H) indicate significant statistical difference compared to controls. Scale bar, 50 μm.