FIGURE

Fig. 4

ID

ZDB-FIG-091026-5

Publication

Hong et al., 2009 - FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1

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Fig. 4

Ier2 and Fibp1 are required to establish left–right asymmetry in zebrafish. Control MO (A, E, I, and M), ier2 MO, 6.5 ng (B, F, J, and N), fibp1 MO, 7 ng (C, G, K, and O), and ier2 MO plus fibp1 MO, 2 plus 3 ng (D, H, L, and P) were injected, and embryos examined by whole-mount in situ hybridization. (Q) Quantification of experiments illustrated in A-L. (A–D) Dorsal view of spaw expression at 19 hpf. MO-injected embryos show bilateral expression (B–D) compared with left side expression of controls (A). (E–H) Ventral view of cmlc2 expression at 36 hpf. Ier2 and fibp1 MO cause abnormal heart laterality and looping (F–H); control in E. (I–L) Asymmetric expression of foxa3 in gut was altered by ier2 or fibp1 MO injection (J–L), compared with controls (I), all at 36 hpf. (M–P) Ier2 and fibp1 MOs caused notochord undulation at 22 hpf. h, heart; g, gut; n, notochord.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-fibpb AB + MO1-ier2a AB + MO1-fibpb + MO1-ier2a
Knockdown Reagents:	MO1-fibpb MO1-ier2a
Observed In:	determination of left/right symmetry notochord
Stage Range:	20-25 somites to Prim-25

Phenotype Detail

Acknowledgments

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