FIGURE

Fig. 3

ID

ZDB-FIG-090804-15

Publication

Yamauchi et al., 2009 - Fgf4 is required for left-right patterning of visceral organs in zebrafish

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Fig. 3

The expression of Fgf4 in zebrafish. (A) The expression of Fgf4 in embryos at 0–4 hpf was examined by RT-PCR. The expression of Fgf19 and ef1α was also examined as negative and positive controls, respectively. Fgf4 was expressed maternally. (B) The expression of Fgf4 in embryos at an early blastula stage, the128-cell stage, was examined by whole mount in situ hybridization (a). The expression of Fgf4 and lrdr1, a marker gene for Kupffer′s vesicle, in embryos at an early stage of segmentation (12 hpf) was also examined by whole mount in situ hybridization. Fgf4 was predominantly expressed in the posterior notochord and Kupffer′s vesicle and its peripheral region. Arrows indicate Kupffer′s vesicle (b–e). Fgf4 was also expressed in the MHB, pharyngeal arch and tail bud at 24 hpf (f) and in the MHB, fin buds and somites at 48 hpf (g). Lateral views (b, d, f, g); dorsal views (c, e). Scale bars = 200 μm.

Expression Data

Genes:	dnah9 fgf4 fgf19
Fish:	WT
Anatomical Terms:	fin bud Kupffer's vesicle midbrain hindbrain boundary notochord posterior region pharyngeal arch somite tail bud whole organism
Stage Range:	1-cell to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 332(1), Yamauchi, H., Miyakawa, N., Miyake, A., and Itoh, N., Fgf4 is required for left-right patterning of visceral organs in zebrafish, 177-185, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.