FIGURE

Fig. 7

ID

ZDB-FIG-090717-78

Publication

Huang et al., 2009 - Discovery of a novel prolactin in non-mammalian vertebrates: evolutionary perspectives and its involvement in teleost retina development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Phenotypic change in the zebrafish eye after knockdown of zfPRL2.

Expression of Isl-1 (A–F), Pax6 (G and I) and Six3 (H and J) in the zebrafish retina was studied before and after zfPRL2 MO knockdown. Isl-1 expression, which is present in the GCL and INL of control MO embryos at 48 hpf (A) and 72 hpf (B), is largely reduced in the INL of zfPRL2 MO embryos (C and D). This reduction of expression in the INL can be rescued by injection of zfPRL2 mRNA (E and F). Pax6 (compare G and I) and Six3 (compare H and J) expression is however not affected at 48 hpf after zfPRL2 MO knockdown. After injection of zfPRL2MO^s-s, the transcription of zfPRL2 was compromised as demonstrated by the aberrant RT-PCR results (K). PRL2 is expressed after 4 hpf in zebrafish embryos (L).

Expression Data

Genes:	isl1a pax6a prl2 six3a
Fish:	WT WT + MO2-prl2
Knockdown Reagent:	MO2-prl2
Anatomical Terms:	retinal ganglion cell layer retinal inner nuclear layer whole organism
Stage Range:	1-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One