ZFIN Image: Huang et al., 2009, Fig. 7

Image

Figure Caption

Fig. 7 Phenotypic change in the zebrafish eye after knockdown of zfPRL2.

Expression of Isl-1 (A–F), Pax6 (G and I) and Six3 (H and J) in the zebrafish retina was studied before and after zfPRL2 MO knockdown. Isl-1 expression, which is present in the GCL and INL of control MO embryos at 48 hpf (A) and 72 hpf (B), is largely reduced in the INL of zfPRL2 MO embryos (C and D). This reduction of expression in the INL can be rescued by injection of zfPRL2 mRNA (E and F). Pax6 (compare G and I) and Six3 (compare H and J) expression is however not affected at 48 hpf after zfPRL2 MO knockdown. After injection of zfPRL2MO^s-s, the transcription of zfPRL2 was compromised as demonstrated by the aberrant RT-PCR results (K). PRL2 is expressed after 4 hpf in zebrafish embryos (L).

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Expression / Labeling details

Acknowledgments

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