Fig. 7

Depletion of ttll3 Expression Produces Defects in the LR Asymmetry in Zebrafish

Ventral views of 22 hpf embryos stained for expression of lefty1 (A–D), or 33 hpf embryos stained for expression of cmlc2 (E–H). Insets in (A)–(D) depict 4x magnifications of the heads of embryos. In all cases, the left side of the embryo is to the left of the panel. lefty1 is normally expressed on the left side of the epiphysis (A). ttll3 morphant embryos express lefty1 on the left body side (B), or display bilateral (C) or right-sided expression (D). Arrows mark the epiphysis. In untreated embryos, cmlc2 (E) is expressed in the myocardium, which is normally located in the left lateral plate. The position of the heart field is unaltered in some TTLL3 morphants (F). Other morphants display midline (G) or right-side expression (H). Arrows in (E)–(H) mark the heart field. (I and J) Cilia in KV stained with 6-11 B-1 anti-acetylated K40 α-tubulin mAb in a control (I) and a TTLL3-ATG morphant (J) at 8-10 somite stage at 14 hpf. (K) A graph represents the average length of cilia in KV in control (n = 3) and TTLL3-ATG morphants (n = 3). Data are presented as means ± SEM.