FIGURE

Fig. 11

ID

ZDB-FIG-090519-36

Publication

Webster et al., 2009 - O-GlcNAc modifications regulate cell survival and epiboly during zebrafish development

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Fig. 11

Pou5f1 is modified by O-GlcNAc in human embryonic stem cells. Western blot of protein immunoprecipitated by an anti-Pou5f1 antibody. The RL2 antibody recognizes a 43 KDa, O-GlcNAc modified protein that is immunoprecipitated from the nucleocytosolic fraction of human ES cells (line BG02) by an anti-Pou5f1 antibody. The immunoprecipitation was specific, since the nucleocytosolic fraction was precleared with a mixture of IgG and A/G PLUS agarose prior to incubation with the anti-Pou5f1 antibody. The anti-Pou5f1 antibody recognizes the same band when the blot is stripped and reprobed, confirming that Pou5f1 protein is modified by O-GlcNAc.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.