ZFIN Figure: Ochi et al., 2009, Fig. 6

Fig. 6

Expression of thin and thick myofilament genes is downregulated by interference with Lbx2 activity. A-J: Analysis of myofilament gene expression. Expression of acta1 (A), tnnt1 (B), tpma (C), tnnt3b (D), tnnc (E), myhz1 (F), mybpc1 (G), smyhc (H), myhz2 (I), mylz2 (J) in control, lbx2-MO injected, lbx2 mRNA injected, and Engrailed suppresser domain fused lbx2 (EnR-lbx2) mRNA injected embryos. Expression of acta1 (A, ctrl: 28/28, lbx2-MO: 0/33, lbx2-mRNA: 9/10, EnR-lbx2 mRNA: 8/8, numerator indicates number of embryos with normal expression, and denominator indicates number of examined embryos), tnnt1 (B, ctrl: 19/19, lbx2-MO: 7/22, lbx2-mRNA: 12/12, EnR-lbx2 mRNA: 17/17), tpma (C, ctrl: 16/16, lbx2-MO: 2/20, lbx2-mRNA: 13/14, EnR-lbx2 mRNA: 10/11) and tnnt3b (D, ctrl: 13/13, lbx2-MO: 0/7, lbx2-mRNA: 7/7, EnR-lbx2 mRNA: 4/4) is reduced by lbx2-MO, but not tnnc (E, ctrl: 17/17, lbx2-MO: 18/22, lbx2-mRNA: 10/10, EnR-lbx2 mRNA: 16/16). Expression of myhz1 (F, ctrl: 25/25, lbx2-MO: 3/16, lbx2-mRNA: 7/8, EnR-lbx2 mRNA: 8/8), mybpc1 (G, ctrl: 15/15, lbx2-MO: 10/20, lbx2-mRNA: 13/14, EnR-lbx2 mRNA: 5/8) and myhz2 (I, ctrl: 17/17, lbx2-MO: 0/5, lbx2-mRNA: 9/9, EnR-lbx2 mRNA: 8/8) is reduced by lbx2-MO, but smyhc (H, ctrl: 36/36, lbx2-MO: 24/29, lbx2-mRNA: 8/8, EnR-lbx2 mRNA: 12/12) and mylz2 are unaltered (J, ctrl: 15/15, lbx2-MO: 14/15, lbx2-mRNA: 10/10, EnR-lbx2 mRNA: 7/7). No obvious difference can be detected between embryos injected with lbx2 mRNA and embryos injected with EnR-lbx2 mRNA. (A-J) Whole mounts, lateral views, rostral toward the left, dorsal toward the top. Scale bar: 100 μm.

Expression Data

Genes:	acta1a mybpc1 myhz1.1 myhz2 mylpfa smyhc1 tnnc2.2 tnnt1 tnnt3b tpma
Fish:	WT WT + MO2-lbx2
Knockdown Reagent:	MO2-lbx2
Anatomical Terms:	fast muscle cell striated muscle myosin thick filament fast muscle cell striated muscle thin filament slow muscle cell striated muscle myosin thick filament slow muscle cell striated muscle thin filament
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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