FIGURE

Fig. 7

ID

ZDB-FIG-090306-8

Publication

Sukumaran et al., 2009 - Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants

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Fig. 7

Immunohistochemical analysis of wild type, ift57, ift88 and ift172 mutant zebrafish at 72 hpf. (A–D) 1D1 (green), a marker for rhodopsin, localized to the outer segment region in wild type and ift57 mutants. Strong mislocalization to the inner segment was observed in ift57 mutants, as well as ift88, and ift172 mutants. (E–H) Zpr1 (green), a label for red/green double cones gave an elongated, columnar morphology in wild type animals. The columnar morphology was disrupted in the three mutants. (I–J) Blue opsin (BOPS) was localized to putative outer segments of wild type photoreceptors, although some mislocalization was seen. Staining was observed in small the apical region of IFT mutant photoreceptors but strong mislocalization was seen in the ift88 and ift172 mutants. (M–P) IFT52 (red) co-localized with acetylated tubulin (green) in the connecting cilia of wild type (arrows). No such staining was observed IFT mutants. (Q–T) IFT88 (red) also showed strong apical localization in wild type embryos (arrows). The staining partially overlapped with acetylated tubulin (green). No staining was seen in the IFT mutants. (U–X) The basal body marker centrin (red) localized to the apical surface of the inner segment. Acetylated tubulin (green) denotes microtubules. In all images, the tissues were counterstained with DAPI (blue). Scale bar = 20 μm.

Expression Data

Antibodies:	Ab1-ift52 Ab1-ift88 Ab1-opn1sw2 Ab1-rho zpr-1
Fish:	WT ift57^{hi3417Tg/hi3417Tg} ift88^tz288/tz288 ift172^{hi2211Tg/hi2211Tg}
Anatomical Terms:	eye photoreceptor cell eye photoreceptor cell apical region eye photoreceptor cell photoreceptor connecting cilium eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment eye photoreceptor cell plasma membrane retinal cone cell
Stage Range:	Pec-fin to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ift57^{hi3417Tg/hi3417Tg} ift88^tz288/tz288 ift172^{hi2211Tg/hi2211Tg}
Observed In:	retinal cone cell
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Vision Research, 49(4), Sukumaran, S., and Perkins, B.D., Early defects in photoreceptor outer segment morphogenesis in zebrafish ift57, ift88 and ift172 Intraflagellar Transport mutants, 479-489, Copyright (2009) with permission from Elsevier. Full text @ Vision Res.