Fig. 7

Fig. 7 Immunohistochemical analysis of wild type, ift57, ift88 and ift172 mutant zebrafish at 72 hpf. (A–D) 1D1 (green), a marker for rhodopsin, localized to the outer segment region in wild type and ift57 mutants. Strong mislocalization to the inner segment was observed in ift57 mutants, as well as ift88, and ift172 mutants. (E–H) Zpr1 (green), a label for red/green double cones gave an elongated, columnar morphology in wild type animals. The columnar morphology was disrupted in the three mutants. (I–J) Blue opsin (BOPS) was localized to putative outer segments of wild type photoreceptors, although some mislocalization was seen. Staining was observed in small the apical region of IFT mutant photoreceptors but strong mislocalization was seen in the ift88 and ift172 mutants. (M–P) IFT52 (red) co-localized with acetylated tubulin (green) in the connecting cilia of wild type (arrows). No such staining was observed IFT mutants. (Q–T) IFT88 (red) also showed strong apical localization in wild type embryos (arrows). The staining partially overlapped with acetylated tubulin (green). No staining was seen in the IFT mutants. (U–X) The basal body marker centrin (red) localized to the apical surface of the inner segment. Acetylated tubulin (green) denotes microtubules. In all images, the tissues were counterstained with DAPI (blue). Scale bar = 20 μm.