FIGURE

Fig. 1

ID

ZDB-FIG-090306-39

Publication

Matthews et al., 2009 - TNF{alpha}-dependent hepatic steatosis and liver degeneration caused by mutation of zebrafish s-adenosylhomocysteine hydrolase

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Hepatic steatosis and liver degeneration in dtp zebrafish larvae. (A-D) Lateral views of 5-dpf wild-type (wt, A,C) and dtp mutant (B,D) larvae showing small liver (outlined, arrow) and reduced yolk consumption (y) in dtp. (C,D) RNA in situ hybridization for the liver marker transferrin (tfa) demonstrates smaller liver size (arrow) in dtp. (E-J) Liver histology showing irregular dtp hepatoctye nuclei beginning at 3 dpf (compare F with E) and with cellular degeneration that is pronounced at 5 dpf (compare J with I). (K,L) Oil Red O (ORO) staining reveals neutral lipid in the small liver of a 5-dpf dtp larva (L) but not in the wild type (K). (M-P) Electron micrographs of the enlarged nuclei (compare N with M, arrowheads), mitochondrial defects (compare P with O, m) and lipid droplets (P, arrows) in 5-dpf dtp hepatocytes. c, canaliculus; eso,esophagus; liv, liver.

Expression Data

Gene:	tfa
Fish:	WT ahcy^p14nb/p14nb
Anatomical Term:	liver
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ahcy^p14nb/p14nb
Observed In:	hepatocyte mitochondrial crista hepatocyte mitochondrion liver liver lipid droplet liver nucleus
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development