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Fig. 1 Hepatic steatosis and liver degeneration in dtp zebrafish larvae. (A-D) Lateral views of 5-dpf wild-type (wt, A,C) and dtp mutant (B,D) larvae showing small liver (outlined, arrow) and reduced yolk consumption (y) in dtp. (C,D) RNA in situ hybridization for the liver marker transferrin (tfa) demonstrates smaller liver size (arrow) in dtp. (E-J) Liver histology showing irregular dtp hepatoctye nuclei beginning at 3 dpf (compare F with E) and with cellular degeneration that is pronounced at 5 dpf (compare J with I). (K,L) Oil Red O (ORO) staining reveals neutral lipid in the small liver of a 5-dpf dtp larva (L) but not in the wild type (K). (M-P) Electron micrographs of the enlarged nuclei (compare N with M, arrowheads), mitochondrial defects (compare P with O, m) and lipid droplets (P, arrows) in 5-dpf dtp hepatocytes. c, canaliculus; eso,esophagus; liv, liver.