FIGURE

Fig. 4

ID

ZDB-FIG-090224-31

Publication

Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression

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Fig. 4

Tcf3 controls the rate of spinal progenitor proliferation. (A-F) The mitotic index (A-C) and the BrdU-labeling index (D-F) of tcf3 morphant (MO) embryos are slightly decreased, yet significantly different (^*), as compared with wild-type (WT) embryos. Error bars show s.e.m. and P-values were calculated using unpaired t-tests. (B,C) Whole-mount views of anti-pH3 staining, with the spinal cord demarcated by the black lines. (E,F) Cross-sections, with BrdU staining in green and propidium iodide (PI) counterstaining in red. (G-J) In situ hybridization on cross-sections shows that proliferative cell nuclear antigen (pcna) and cdkn1c (p57) are expressed normally in tcf3 morphants at 18 hpf.

Expression Data

Genes:	cdkn1ca pcna
Fish:	WT WT + MO2-tcf7l1a + MO3-tcf7l1b
Knockdown Reagents:	MO2-tcf7l1a MO3-tcf7l1b
Anatomical Term:	spinal cord neural tube
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-tcf7l1a + MO3-tcf7l1b
Knockdown Reagents:	MO2-tcf7l1a MO3-tcf7l1b
Observed In:	cell population proliferation spinal cord neuroblast (sensu Vertebrata)
Stage:	14-19 somites

Phenotype Detail

Acknowledgments

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