FIGURE

Fig. 5

ID

ZDB-FIG-090223-21

Publication

Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations

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Fig. 5

Mrf4 has no role in trunk muscle differentiation. (A) Schematic illustrating the location of the mutation encoded by mrf4^hu2041. (B) Proportion of mrf4^hu2041^/^hu2041 (red), mrf4^hu2041^/+ (green) or mrf4⁺^/+ (blue) in progeny from heterozygote crosses. The number of embryos of each genotype is shown. Homozygote mutants survive well and without obvious defects. (C) Whole-mount in situ mRNA hybridisation of myf5+myod morphants or myod MO-injected myf5^hu2022 mutants reveal that residual medial fast fibres express myog (red) but not mrf4 (purple) at 22 ss, but accumulate mrf4 mRNA after terminal differentiation at 24 hpf. Lateral view, anterior to left. (D) In situ mRNA hybridisation for myhz1 (purple) in embryos from a mrf4^+/hu2041 incross reveals that injection of myf5+myod MOs fails to ablate all muscle irrespective of mrf4 genotype.

Expression Data

Genes:	myf6 myhz1.1 myog
Fish:	WT myf5^{hu2022/hu2022} myf6^{hu2041/hu2041} WT + MO1-myod1 myf5^{hu2022/hu2022} + MO1-myod1 WT + MO1-myod1 + MO3-myf5 myf6^{hu2041/hu2041} + MO1-myod1 + MO3-myf5
Knockdown Reagents:	MO1-myod1 MO3-myf5
Anatomical Terms:	fast muscle cell muscle cell somite
Stage Range:	20-25 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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