FIGURE

Fig. S4

ID

ZDB-FIG-090220-71

Publication

Carreira-Barbosa et al., 2009 - Flamingo regulates epiboly and convergence/extension movements through cell cohesive and signalling functions during zebrafish gastrulation

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Fig. S4

Celsr-ΔC causes C&E and epiboly defects, and injection of a low dose of stbmMO, dvl2MO or Lyn-Celsr causes C&E but not epiboly defects in MZord embryos. MZord embryos (A,D) or MZord embryos injected with 0.4 pmoles stbmMO (B,E), 0.75 pmoles dvl2MO (C,F) or 20 pg Lyn-Celsr RNA (I,L). Wild-type embryos were injected with 300 pg Celsr-ΔC-HA RNA (H,K) or left uninjected (G,J). The embryos were fixed at tail-bud and subjected for in situ hybridisation with hgg1 for the prechordal plate, ntl for the notochord and dlx3 for the anterior edge of the neural plate. The results of C&E phenotypes are summerised in Table S1. Note that injection of 300 pg Celsr-ΔC-HA RNA causes C&E defects with posteriorly misplaced prechordal plate as well as epiboly defects shown by the shifted germ ring expression of ntl (indicated by an arrowhead).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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