Fig. 6

Lyn-Celsr inhibits Frizzled-induced membrane localisation of Dishevelled through a highly conserved SE/D domain. (A) Schematic illustration of the intracellular region of Fmi/Celsr. Two domains are conserved among vertebrates as highlighted in blue. Only one of these, the SD/E domain is conserved between vertebrates and Drosophila. The partial sequence of a construct lacking this domain is shown. (B-M) Wild-type embryos injected with 150 pg Dsh-GFP RNA in the absence (B-D) or presence (E-M) of 100 pg Fz7 RNA, together with 100 pg membrane-RFP (E-G), Lyn-Celsr-RFP (B-D,H-J) or Lyn-Celsr-Δ-RFP (K-M), as indicated and analysed at 40% epiboly by confocal microscopy. Scanning for GFP and RFP was carried out simultaneously and merged (D,G,J,M). Fz7-mediated membrane localisation of Dsh is inhibited by Lyn-Celsr (H,J) but not by Lyn-Celsr-Δ (K,M), which lacks the SD/E domain conserved between vertebrates and Drosophila (A).