Fig. 7

Wnt5b uniquely modifies midline bifurcation incidence; Wnt11 uniquely modifies cyclopia incidence. (A–F) Phenotypes of Wnt5b MO-injected embryos. Injection of a control MO did not cause obvious defects in either WT embryos (A), MZsqt embryos (C) or MZoep embryos (E). Injection of 6 ng of a Wnt5b MO caused shortened tails in WT (B) and MZsqt embryos (D); however, it also caused MB in MZsqt embryos (D) and MZoep embryos (F). (G) Quantification of increased MB in Wnt5b MO-injected MZsqt and MZoep embryos. Significant differences were observed in the MB rates of control MO versus Wnt5b MO-injected MZsqt embryos (p = 0.00005) and control MO versus Wnt5b MO-injected MZoep embryos (p = 0.04068), but no significant differences were seen in the cyclopia rates of control MO versus Wnt5b MO-injected MZsqt embryos (p = 0.90922), and all MZoep embryos had cyclopia. (H) Quantification of increased cyclopia in Wnt11 MO-injected MZsqt and WT embryos. Significant differences were observed in the cyclopia rate of control MO versus Wnt11 MO-injected MZsqt (p = 0.0002) and control MO versus Wnt11 MO-injected WT (p = 0.00066) embryos, but MB was not seen in any Wnt11 MO injected embryos. Numbers of embryos scored are shown and embryo percentages are indicated as follows: normal appearing (white); cyclopia alone (green); MB alone (red) and MB + cyclopia (yellow).