PUBLICATION
Identification of common and unique modifiers of zebrafish midline bifurcation and cyclopia
- Authors
- Pei, W., and Feldman, B.
- ID
- ZDB-PUB-081203-33
- Date
- 2009
- Source
- Developmental Biology 326(1): 201-211 (Journal)
- Registered Authors
- Feldman, Benjamin, Pei, Wuhong
- Keywords
- none
- MeSH Terms
-
- Animals
- Body Patterning/physiology
- Embryo, Nonmammalian/abnormalities
- Eye Abnormalities/embryology*
- Gastrulation/physiology*
- HSP90 Heat-Shock Proteins/genetics
- HSP90 Heat-Shock Proteins/metabolism
- Hot Temperature
- Nodal Signaling Ligands/genetics
- Nodal Signaling Ligands/metabolism
- Wnt Proteins/metabolism
- Zebrafish/abnormalities*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 19046963 Full text @ Dev. Biol.
Citation
Pei, W., and Feldman, B. (2009) Identification of common and unique modifiers of zebrafish midline bifurcation and cyclopia. Developmental Biology. 326(1):201-211.
Abstract
Loss of the zebrafish Nodal-related protein Squint causes a spectrum of phenotypes including cyclopia and midline bifurcations (MB). Here we examine MBs and their relation to cyclopia in maternal-zygotic squint (MZsqt) mutants. There is a concordance of MB with cyclopia in MZsqt embryos, and heat treatment or depletion of Hsp90a is a "common" risk factor that increases the incidence of both phenotypes. Midline identity is specified on both sides of MBs, and deep-layer cells are initially lacking within bifurcations, whereas enveloping layer cells are intact. Bifurcations do not appear until the completion of gastrulation and are preceded by gaps in the expression of wnt5b, an essential regulator of dorsal convergence. The incidence of early MBs and wnt5b expression defects in heated MZsqt embryos is high, but there is also substantial recovery. Wnt5b depletion increases the incidence of MB, but not cyclopia, and as such Wnt5b is a "unique" risk factor for MB. Reciprocally, depletion of Wnt11 or Hsp90b increases cyclopia only. In summary, we find that MB arises after gastrulation in regions that fail to express wnt5b, and we show that two complex dysmorphologies - MB and cyclopia - can be promoted by either common or unique risk factors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping