FIGURE

Fig. 7

ID

ZDB-FIG-090127-26

Publication

Targoff et al., 2008 - Nkx genes regulate heart tube extension and exert differential effects on ventricular and atrial cell number

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Fig. 7

nkx genes are necessary to establish sufficient ventricular cell number during cardiac chamber formation. (A,B) Immunofluorescence indicates that both chambers express the transgene Tg(cmlc2:DsRed2-nuc) (red), facilitating cardiomyocyte counting at 52 hpf. Atria are labeled with the anti-Amhc antibody, S46 (green). Hearts are flattened with a cover slip to improve visualization of cardiomyocyte nuclei. (C) Quantification of cardiomyocyte nuclei in wild-type (n = 10) and nkx-deficient (n = 13) embryos reveals a statistically significant increase in atrial cell number in nkx-deficient embryos (p < 0.001, Student's t-test) and a statistically significant decrease in ventricular cell number in nkx-deficient embryos (p < 0.001, Student's t-test). Bar graph indicates mean and standard error of each data set; asterisks indicate statistically significant differences from wild-type.

Expression Data

Gene:	DsRed2
Antibody:	Ab-S46
Fish:	f2Tg f2Tg + MO1-nkx2.7 + MO2-nkx2.5
Knockdown Reagents:	MO1-nkx2.7 MO2-nkx2.5
Anatomical Terms:	atrium cardiac muscle cell cardiac ventricle cardiac muscle cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	f2Tg + MO1-nkx2.7 + MO2-nkx2.5
Knockdown Reagents:	MO1-nkx2.7 MO2-nkx2.5
Observed In:	atrial myocardium cardiac muscle cell atrium cardiac ventricle embryonic heart tube development ventricular myocardium cardiac muscle cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 322(2), Targoff, K.L., Schell, T., and Yelon, D., Nkx genes regulate heart tube extension and exert differential effects on ventricular and atrial cell number, 314-321, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.