Fig. 4

The effect of loss of Notch receptors on surface accumulation of DeltaD. (A-C′) A comparison of DeltaD distribution in wild type, notch1a and notch3 morphants, and mib^m178 mutant zebrafish embryos at the tail bud stage. Areas shown here approximately correspond to rectangular areas in Fig. S1A-D (see supplementary material). Embryos are double-stained with zdD2 (grey in A-C, green in A′-C′) and β-catenin (red in A′-C′). DeltaD-expressing cells are increased and DeltaD is mainly localized at cell surface in both Notch morphants (B,B′) and mib^m178 mutants (C,C′). (D-F′) DeltaD distribution in Notch morphants with (F,F′) or without (E,E′) co-injection of deltaA and deltaD (deltaA&D) MOs. Though co-injection of deltaA&D MO dramatically reduces zdD2 staining (compare E with F), DeltaD is still localized at the cell surface.