FIGURE

Fig. 3

ID

ZDB-FIG-090113-23

Publication

Matsuda et al., 2009 - Interaction with Notch determines endocytosis of specific Delta ligands in zebrafish neural tissue

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Fig. 3

The effect of loss of Notch signaling on surface accumulation of DeltaD. DeltaD distribution in DMSO-treated (A,A′), DAPT-treated (B,B′) or DN-Su(H)-expressing zebrafish embryos (C,C′) at the tail bud stage, and in DMSO-treated (D,D′) and DAPT-treated (E,E′)embryos at the six-somite stage (6 ss). Areas shown in A-C′ correspond approximately to rectangular areas in Fig. S1E,F (see supplementary material). Embryos were stained with zdD2 (A-E) and double-stained (A′-E′) with β-catenin (zdD2 in green, β-catenin in red). DAPT and DN-Su(H) treatment increased the numbers of cells expressing DeltaD but did not result in DeltaD accumulation at the cell surface at the tail bud stage. Some cell surface DeltaD is detected in DAPT-treated embryos by the six-somite stage; however, it is accompanied by intense cytoplasmic puncta.

Expression Data

Antibodies:	Ab1-dld Ab3-ctnnb
Fish:	WT
Condition:	chemical treatment: DAPT
Anatomical Terms:	hindbrain neural plate adherens junction hindbrain neural plate cell surface hindbrain neural plate cytoplasm
Stage Range:	Bud to 5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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