FIGURE

Fig. S4

ID

ZDB-FIG-081222-11

Publication

Osborne et al., 2008 - The spinster homolog, two of hearts, is required for sphingosine 1-phosphate signaling in zebrafish

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Fig. S4

Fibronectin Expression Is Affected in mil MO-Injected Embryos Confocal micrographs of cross sections of uninjected (A and A′) and injected with 2 ng of mil MO (B and B′) Tg(-0.7her5:EGFP)^ne2067 embryos (GFP false colored blue) stained for β-catenin (red) to show cellular outlines and Fibronectin (green) at 20 hpf. Control embryos (A and A′) have endodermal cells (blue) spanning the midline (blue) and show strong Fibronectin deposition (green) under the endoderm and around the migrating precardiac mesoderm. mil MO injected embryos (B and B′) show gaps in the endodermal sheet (blue) and have considerably less Fibronectin deposition (green) at the midline (arrowhead), while lateral Fibronectin deposition (arrow) appears relatively unaffected.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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