FIGURE

Fig. 6

ID

ZDB-FIG-081219-34

Publication

Gansner et al., 2008 - Essential role for the alpha 1 chain of type VIII collagen in Zebrafish notochord formation

Other Figures

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Fig. 6

Morpholino knockdown of col8a1 recapitulates the gul^m208 phenotype. A: Wild-type embryos injected with 12 ng of control morpholino exhibit normal morphology. B: Embryos injected with 2.4 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) characteristic of gul^m208 mutants. C: Reverse transcriptase-polymerase chain reaction (RT-PCR) at 24 hours postfertilization (hpf) demonstrating a dose-dependent reduction in col8a1 transcript after morpholino injection. D: Embryos injected with 12 ng of a splice morpholino targeting col8a1 develop notochord distortion (arrowheads) that is worse than in gul^m208 mutants. Insets show notochord at higher magnification. All embryos were treated with PTU to inhibit melanin pigmentation and photographed at 30 hpf.

Expression Data

Genes:	col8a1a tbx16
Fish:	WT WT + MO2-col8a1a
Knockdown Reagent:	MO2-col8a1a
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-col8a1a
Knockdown Reagent:	MO2-col8a1a
Observed In:	notochord notochord formation
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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