FIGURE

Fig. 2

ID

ZDB-FIG-081219-31

Publication

Gansner et al., 2008 - Essential role for the alpha 1 chain of type VIII collagen in Zebrafish notochord formation

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Fig. 2

Electron microscopy reveals notochord abnormalities in gul^m208 mutants. A-E: Transmission electron micrographs of truncal cross-sections from embryos at 24 hpf. A: Notochord sheath and vacuolated notochord cell of a wild-type embryo, with small areas of protein accumulation visible in the rough endoplasmic reticulum (arrow). B: Notochord sheath and vacuolated notochord cell of a gul^m208 mutant with large circular aggregates of protein in the rough endoplasmic reticulum (arrow). C: Notochord and hypochord of a gul^m208 mutant with large circular aggregrates of protein in the hypochord (red arrows) and notochord (yellow arrow). D: Notochord sheath of a wild-type embryo with inner (i), medial (m), and outer (o) layers indicated. Small areas of protein accumulation are visible in the rough endoplasmic reticulum (red arrow). E: Notochord sheath of a gul^m208 mutant where the collagen fibrils in the medial (m) layer appear disorganized. A large circular aggregate of protein is visible in the rough endoplasmic reticulum (red arrow). Not, vacuolated notochord cell; Hyp, hypochord.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	col8a1a^m208/m208
Observed In:	hypochord rough endoplasmic reticulum hypochord development notochord notochord rough endoplasmic reticulum notochord formation protein secretion
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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