FIGURE

Fig. 3

ID

ZDB-FIG-081117-43

Publication

Bessarab et al., 2008 - Six1a is required for the onset of fast muscle differentiation in zebrafish

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Fig. 3

Six1a is required for early myog expression in fast muscle precursors. Flat mount embryos after two-color WISH for myog (purple) and mespa (red) at 9 s (A, B) or single-color in situ hybridization for myog (A′, B′, C, and D), smyhc1 (E–G) or myod (H, I) at 9 s. The four posterior somites with the adjacent presomitic mesoderm are shown. Insets show the posterior part of the embryo. Arrowheads indicate the region of fast muscle precursors. Note that the myog expression is nearly absent along the entire posterior border in SIII and retained in a few medially located cells in SIV (B) and in somites shown in panel D (white arrowheads). The expression in the adaxial cells (white arrow) was retained (B and D). The hardly visible residual myog expression in the affected cells marked by asterisks. CM caused no effect on myog expression (B′). UM or SM cause no obvious effect on the expression of slow muscle-specific marker smyhc1 (F and G, 62/65 and 55/60 of embryos injected with UM or SM, correspondingly) or on myod expression in fast muscle precursors (I; 70/73 of embryos injected with SM). Scale bar, 100 μm.

Expression Data

Genes:	mespaa myod1 myog smyhc1
Fish:	AB AB + MO4-six1b AB + MO5-six1b
Knockdown Reagents:	MO4-six1b MO5-six1b
Anatomical Terms:	adaxial cell fast muscle cell segmental plate slow muscle cell somite
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO4-six1b AB + MO5-six1b
Knockdown Reagents:	MO4-six1b MO5-six1b
Observed In:	somitomeric trunk muscle development striated muscle cell differentiation
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 323(2), Bessarab, D.A., Chong, S.W., Srinivas, B.P., and Korzh, V., Six1a is required for the onset of fast muscle differentiation in zebrafish, 216-228, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.