FIGURE

Fig. 6

ID
ZDB-FIG-080923-7
Publication
Tallafuss et al., 2008 - The Met receptor tyrosine kinase prevents zebrafish primary motoneurons from expressing an incorrect neurotransmitter
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Met is required for secondary motoneuron formation. (a-b′) gata2:GFP (green) transgenic embryos at 48 hpf labeled with antibody to Alcam (red). gata2:GFP is expressed in ventrally projecting SMNs, some of which also express Alcam (a); (a′) shows only the GFP. met MO-injected embryos (b,b′) show a severe decrease in SMNs, but Alcam labeling of the floor plate appears normal. A small number of SMNs form and make ventral projections (arrow). (b) Both markers shown; (b′) only GFP shown. (c,c′). Some of the GFP-positive cells in met MO-injected embryos show interneuron-like axons. (c) An interneuron with an ascending axon; (c′) an interneuron with a descending axon. The two GFP-positive cells to the right in (c′) are SMNs. (d-g) gata2:GFP transgenic embryos (green) at 48 hpf labeled with Alcam antibody (red). Exposure to SB203580 (f) severely decreased the formation of SMNs relative to controls (d), but the overall architecture of the spinal cord appeared normal based on expression of GFP driven by the pax2a promoter (e,g). Scale bar, 20 μm in all panels except (c,c′), which is 10 μm.

Expression Data
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Neural Dev.