FIGURE

Fig. 4

ID

ZDB-FIG-080923-5

Publication

Tallafuss et al., 2008 - The Met receptor tyrosine kinase prevents zebrafish primary motoneurons from expressing an incorrect neurotransmitter

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Fig. 4

Met appears unnecessary for muscle and neuromuscular junction formation. (a,b) Engrailed antibody (Eng, red) labeling showing muscle pioneer cells and znp1 antibody labeling showing motor axons (green). In met MO-injected embryos, some CaP axons are truncated (asterisk). (c,d) F59 antibody (red) labeling showing fast muscle fibers and znp1 antibody staining showing motor axons (green). F59 labeling appears the same in control (c) and met MO-injected (d) embryos, which have some truncated CaPs (asterisk). (e-f) αBTX (red) labeling showing AChRs and znp1 antibody labeling showing motor axons (green). The distribution of AChRs appears the same in control (e) and met MO-injected embryos (f) that have some truncated CaP axons (asterisks); however, it appears that the number of AChRs may be decreased at the myoseptal varicosity (arrows) by MO injection. For each experiment, 8 spinal hemisegments plus somites were examined in each of 21–33 met MO-injected embryos and 8 spinal hemisegments plus somites in each of 15 controls. Scale bar, 20 μm.

Expression Data

Antibodies:	Ab-F59 Ab1-eng znp-1
Fish:	WT WT + MO1-met + MO2-met
Knockdown Reagents:	MO1-met MO2-met
Anatomical Terms:	fast muscle cell motor neuron axon muscle pioneer
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-met + MO2-met
Knockdown Reagents:	MO1-met MO2-met
Observed In:	CaP motoneuron axon
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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