FIGURE

Fig. 6

ID

ZDB-FIG-080909-41

Publication

Iwanami et al., 2008 - WDR55 Is a Nucleolar Modulator of Ribosomal RNA Synthesis, Cell Cycle Progression, and Teleost Organ Development

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Fig. 6

Zebrafish WDR55 mutant shows defect in thymus development.
(A) Retroviral insertion in WDR55 locus of zebrafish hi2786B mutants. Uppercase letters indicate exons with predicted amino acids. Arrow indicates WDR domain. (B) Transcription of WDR55 3′ region and GAPDH in hi2786B (-/-) and heterozygous (+/-) zebrafish larvae at 6 dpf. cDNA was synthesized in the absence (-) or presence (+) of reverse transcriptase (RT). (C) Phenotypes of WT (top) and hi2786B (bottom) zebrafish. Far left panels show lateral views of 6-dpf larvae. Arrows indicate swim bladder. Second panels from the left show whole-mount in situ hybridization of 6-dpf larvae using rag1 probe. Ventral views are shown. Embryos were treated with H₂O₂ to bleach pigment cells. Middle panels show hematoxylin-eosin (HE) staining of transverse sections at 6 dpf. Broken blue lines indicate the thymus. Second panels from the right show Alcian blue staining of 6-dpf larvae. Ventral views are shown. Numbers indicate pharyngeal arches. Far right panels show HE staining of transverse sections of the eyes at 6 dpf.

Expression Data

Genes:	rag1 wdr55
Fish:	WT wdr55^{hi2786bTg/hi2786bTg} wdr55^hi2786bTg/+
Anatomical Terms:	thymus whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wdr55^{hi2786bTg/hi2786bTg}
Observed In:	eye pharyngeal arch swim bladder thymus
Stage:	Day 6

Phenotype Detail

Acknowledgments

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