ZFIN Image: Iwanami et al., 2008, Fig. 6

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Figure Caption

Fig. 6 Zebrafish WDR55 mutant shows defect in thymus development.
(A) Retroviral insertion in WDR55 locus of zebrafish hi2786B mutants. Uppercase letters indicate exons with predicted amino acids. Arrow indicates WDR domain. (B) Transcription of WDR55 3′ region and GAPDH in hi2786B (-/-) and heterozygous (+/-) zebrafish larvae at 6 dpf. cDNA was synthesized in the absence (-) or presence (+) of reverse transcriptase (RT). (C) Phenotypes of WT (top) and hi2786B (bottom) zebrafish. Far left panels show lateral views of 6-dpf larvae. Arrows indicate swim bladder. Second panels from the left show whole-mount in situ hybridization of 6-dpf larvae using rag1 probe. Ventral views are shown. Embryos were treated with H₂O₂ to bleach pigment cells. Middle panels show hematoxylin-eosin (HE) staining of transverse sections at 6 dpf. Broken blue lines indicate the thymus. Second panels from the right show Alcian blue staining of 6-dpf larvae. Ventral views are shown. Numbers indicate pharyngeal arches. Far right panels show HE staining of transverse sections of the eyes at 6 dpf.

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Expression / Labeling details

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Acknowledgments

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