FIGURE

Fig. S6

ID

ZDB-FIG-080828-30

Publication

Goessling et al., 2008 - APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development

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Fig. S6

The effects of wnt activation on mature endodermal organs persist into the larval stage. (A–I) Confocal microscopy and FACS analysis of live zebrafish larvae at 144 hpf (6 dpf). (A–C) APC^+/^-; lfabp:GFP larvae maintain increased liver size; this is confirmed by FACS analysis. (D–F) The pancreas of APC^+/^-; ptf1α:GFP larvae remains smaller than that of wild-type siblings. (G–H) The effect on the intestine as indicated in ifabp:GFP reporter fish parallels that of the liver. (J) Induction of wnt8 after liver differentiation at 48 hpf increased the number of LFABP:GFP+ hepatocytes at 72 (wild-type 1.25 ± 0.12% vs. wnt8 2.42 ± 0.43%, n = 10, p < 0.0005) and 120 hpf (wild-type 2.17 ± 0.19% vs. wnt8 5.21 ± 0.37%, n = 10, p < 0.0005).

Expression Data

Genes:	DsRed EGFP
Fish:	as3Tg jh1Tg zf129Tg apc^hu745/+; as3Tg apc^hu745/+; jh1Tg apc^hu745/+; zf129Tg
Anatomical Terms:	intestine liver pancreas
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 320(1), Goessling, W., North, T.E., Lord, A.M., Ceol, C., Lee, S., Weidinger, G., Bourque, C., Strijbosch, R., Haramis, A.P., Puder, M., Clevers, H., Moon, R.T., and Zon, L.I., APC mutant zebrafish uncover a changing temporal requirement for wnt signaling in liver development, 161-174, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.