FIGURE

Fig. 2

ID

ZDB-FIG-080826-3

Publication

Heanue et al., 2008 - Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species

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Fig. 2

Ret regulates enteric neuron colonization of the gut in a dose-dependent manner. (A–D) Zebrafish injected with translation blocking antisense morpholino oligos against ret ret MO at the one- to two-cell stage are analyzed for the presence of Hu-expressing enteric neurons at 4dpf. Compared to control morpholino injected fish that possess enteric neurons along the full length of the gut tube (A), zebrafish injected with 6 ng retMO show an absence of enteric neurons throughout the majority of the gut tube (B). While injection of 1 ng retMO has no apparent effect on enteric neurons (C), injection of 3 ng of retMO leads to partial phenotypes, with enteric neurons absent from only the distal most gut regions (compare (D) to (B) and (C)). Asterisk indicates the end of gut tube. Arrows indicate distal most enteric neurons. All panels show lateral views. Images for comparison are photographed at the same magnification.

Expression Data

Antibody:	Ab1-elavl
Fish:	WT WT + MO2-ret
Knockdown Reagent:	MO2-ret
Anatomical Term:	enteric nervous system
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-ret
Knockdown Reagent:	MO2-ret
Observed In:	enteric nervous system neuron enteric nervous system development enteric neuron posterior intestine neuron
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 125(8), Heanue, T.A., and Pachnis, V., Ret isoform function and marker gene expression in the enteric nervous system is conserved across diverse vertebrate species, 687-699, Copyright (2008) with permission from Elsevier. Full text @ Mech. Dev.