FIGURE

Fig. 3

ID

ZDB-FIG-080613-16

Publication

Toyama et al., 2008 - Brd4 associates with mitotic chromosomes throughout early zebrafish embryogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Brd4 protein analysis. A: Antibody characterization. Polyclonal antibody against the Brd4 C-terminal 14 residues detected an approximately 150-kDa protein in zebrafish embryonic extracts (A, lanes 3, 4, 7, 8, and 10). Preimmune serum (pre-imm, lanes 1 and 2) did not detect this protein. Antibody preincubated with antigen peptide (pre-abs, lane 5 and 6) failed to detect the 150-kDa protein. Anti-Brd4 antibody cross-reacts with a protein of predicted size in extracts from Xenopus embryos (lane 9). B: Brd4 protein during zebrafish embryogenesis. Extracts equivalent to two embryos were loaded in each lane. Lower panel shows α-tubulin as a loading control. Stages are indicated for each lane. C: The C-terminal amino acid sequence of zebrafish, Xenopus, and mouse is shown with differences in red.

Expression Data

Gene:	brd4
Antibody:	Ab1-brd4
Fish:	WT
Anatomical Term:	whole organism
Stage Range:	1-cell to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.