FIGURE

Fig. 5

ID

ZDB-FIG-080605-24

Publication

Lyons et al., 2002 - A nonsense mutation in zebrafish gata1 causes the bloodless phenotype in vlad tepes

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Fig. 5

DNA-binding activity of Gata1 and Gata1 (Arg-339 → STOP). (a) Unlabeled in vitro translated proteins, wild-type zebrafish Gata1 (zgata1), zebrafish Gata1 (Arg-339 → Stop) (Arg-Stop), and negative control protein, luciferase (luc), were used in an electrophoretic mobility shift assay with the indicated ³²P-labeled probes, β/ε globin or α1-globin. The DNA-protein complexes are seen as slower migrating bands, marked by an arrowhead. The arrow marks free probe, demonstrating excess of DNA in the mixture. (b) Polyacrylamide gel electrophoresis of in vitro translated proteins generated in the presence of [³⁵S]methionine and visualized by autoradiography.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA